PNAs (blockers for mitochondrial and chloroplast amplification)

• More organelles were filtered from the leaves than the roots, sediment, or water.

• using PNAs did not result in an overall loss of reads

• in progress: do we see more chimeras with PNA use?

Run summary statistics

• 533 samples, including kit controls and PNA tests
• reads per sample (after filtration) ranged from 5 – 206,661
• the kit control with the most reads had 6549
• 306 (57.4%) of the samples had fewer than 6549 reads, we’ll call these low-abundance samples
• breakdown of low-abundance samples by type

• in progress: not sure, I published this post by accident